How much DNA can you get from miniprep?

Deliver high-quality plasmid DNA up to 60 µg with miniprep kits. The GeneJET Plasmid Miniprep Kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure utilizing a silica-based membrane within the spin column.

How much DNA does a MaxiPrep yield?

Norgen’s Plasmid DNA MaxiPrep Kit provides a rapid method for the preparation of large quantities of high quality DNA. Typical yields from a 100 mL cul- ture for a high copy number plasmid are between 0.4 and 1.0 mg, with final endotoxin levels of 0.1 EU/ µg of DNA or less.

What is a good miniprep concentration?

The average DNA concentration obtained with the DirectPrep 96 Miniprep Kit is 66 ng/ul (typically >50 ng/ul) in an eluate volume of 50 ul. Up to 4 ug DNA total can be purified from 1.3 ml of LB culture medium. Please note that the actual yield will depend on the vector and host strain being used.

What is a good DNA concentration ng UL?

The effective read range of UV spectroscopy is 0.1 to 0.999 which corresponds to approximately 4 ng/µL to 50 ng/µL of genomic DNA. Values above or below that range are invalid absorbance readings.

How much bacteria do you need for miniprep?

Small scale purification (miniprep) from less than 5 ml of bacterial culture is a quick way for clone verification or DNA isolation, followed by further enzymatic reactions (polymerase chain reaction and restriction enzyme digestion).

How do you concentrate DNA after miniprep?

FAQ

  1. Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample.
  2. Mix, and store at –20°C for at least 1 h to precipitate the DNA.
  3. Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15–20 min.

How does DNA miniprep work?

Aka ALKALINE LYSIS, “minipreps” are experiments in which we separate and purify the plasmid DNA we put into bacteria from all the stuff that was already in the bacteria. You can’t just break the cells open (lyse them) & pull out all the DNA because the bacteria has its own DNA you aren’t interested in.

How do I increase my miniprep yield?

Some molecular biology laboratory technique websites have suggested that increasing the amount of antibiotic present in E. coli cell cultures, which increases selective pressure for retention of the plasmids, can enhance DNA miniprep yields.

What is a normal DNA concentration?

A ratio of ~1.8 is generally accepted as “pure” for DNA; a ratio of ~2.0 is generally accepted as “pure” for RNA. If the ratio is appreciably lower in either case, it may indicate the presence of protein, phenol or other contaminants that absorb strongly at or near 280nm.